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Faecal bacteriome and metabolome profiles associated with decreased mucosal inflammatory activity upon anti-TNF therapy in paediatric Crohn's disease

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Autor
Hurych, JakubORCiD Profile - 0000-0002-9813-5290Scopus Profile - 57221439347
Mascellani Bergo, Anna
Lerchová, TerezaORCiD Profile - 0000-0003-2260-2347Scopus Profile - 57204115312
Hlináková, LucieScopus Profile - 57222585632
Kubát, Michal
Malcová, HanaScopus Profile - 6506572925
Cebecauerová, DitaScopus Profile - 8598812900
Schwarz, JanORCiD Profile - 0000-0003-4214-8858WoS Profile - O-5989-2017Scopus Profile - 56719099000
Karaskova, Eva
Hecht, Tomáš
Vyhnánek, RadimORCiD Profile - 0000-0001-7796-4201WoS Profile - N-7053-2017Scopus Profile - 42862407300
Toukalkova, Lenka
Dotlačil, VojtěchORCiD Profile - 0000-0002-7291-7323Scopus Profile - 57214316280
Greinerova, Katerina
Cizkova, Anabela
Horváth, RudolfORCiD Profile - 0000-0001-5787-9990Scopus Profile - 14058253100
Bronský, JiříORCiD Profile - 0000-0002-2641-7280Scopus Profile - 6603234228
Havlik, Jaroslav
Hradský, OndřejORCiD Profile - 0000-0001-6193-0488WoS Profile - L-5899-2019Scopus Profile - 24280716400
Cinek, OndřejORCiD Profile - 0000-0002-0526-8714WoS Profile - AAF-6664-2020Scopus Profile - 6603698077

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Datum vydání
2024
Publikováno v
Journal of Crohn's and Colitis
Ročník / Číslo vydání
18 (1)
ISBN / ISSN
ISSN: 1873-9946
Metadata
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Kolekce
  • 1. lékařská fakulta
  • 2. lékařská fakulta
  • Lékařská fakulta v Plzni

Tato publikace má vydavatelskou verzi s DOI 10.1093/ecco-jcc/jjad126

Abstrakt
BACKGROUND AND AIMS: Treatment by anti-TNFα antibodies (anti-TNF) changes the dysbiotic faecal bacteriome in Crohn's disease (CD). However, it is not known whether these changes are due to decreasing mucosal inflammatory activity or whether similar bacteriome reactions might be observed in gut-healthy subjects. Therefore, we explored changes in faecal bacteriome and metabolome upon anti-TNF administration (and therapeutic response) in children with CD and contrasted those to anti-TNF-treated children with juvenile idiopathic arthritis (JIA). METHODS: Faecal samples collected longitudinally before and during anti-TNF therapy were analysed for bacteriome by massively parallel sequencing of the 16S rDNA (V4 region) and for faecal metabolome by 1H nuclear magnetic resonance. The response to treatment by mucosal healing was assessed by MINI index at three months after the treatment started. We also tested several representative gut bacterial strains for in-vitro growth inhibition by infliximab. RESULTS: We analysed 530 stool samples from 121 children (CD 54, JIA 18, healthy 49). Bacterial community composition reacted on anti-TNF in CD: three members of class Clostridia increased on anti-TNF, whereas class Bacteroidia decreased. Among faecal metabolites, glucose and glycerol increased, whereas isoleucine and uracil decreased. Some of these changes differed by treatment response (mucosal healing) after anti-TNF. No significant changes in bacteriome or metabolome were noted upon anti-TNF in JIA. Bacterial growth was not affected by infliximab in a disc diffusion test. CONCLUSIONS: Our findings suggest that gut mucosal healing is responsible for the bacteriome and metabolome changes observed in CD, rather than any general effect of anti-TNF.
Klíčová slova
Crohn’s disease, IBD, anti-TNF, children, metabolomics, microbiome
Trvalý odkaz
https://hdl.handle.net/20.500.14178/2114
Zobraz publikaci v dalších systémech
WOS:001078501800001
SCOPUS:2-s2.0-85183581822
PUBMED:37527838
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