Sequencing-based analysis of clonal evolution of 25 mantle cell lymphoma patients at diagnosis and after failure of standard immunochemotherapy
![Thumbnail](/bitstream/handle/20.500.14178/2001/American%20J%20Hematol%20-%202023%20-%20Karolov%c3%a1%20-%20Sequencing%e2%80%90based%20analysis%20of%20clonal%20evolution%20of%2025%20mantle%20cell%20lymphoma%20patients%20at.pdf.jpg?sequence=5&isAllowed=y)
Autor
Tušková, Liliana
Benešová, Kateřina
Dolníková, Alexandra
Svobodová, Karla
Krkavcová, Eva
Datum vydání
2023Publikováno v
American Journal of HematologyRočník / Číslo vydání
98 (10)ISBN / ISSN
ISSN: 0361-8609Metadata
Zobrazit celý záznamTato publikace má vydavatelskou verzi s DOI 10.1002/ajh.27044
Abstrakt
Our knowledge of genetic aberrations, that is, variants and copy number variations (CNVs), associated with mantle cell lymphoma (MCL) relapse remains limited. A cohort of 25 patients with MCL at diagnosis and the first relapse after the failure of standard immunochemotherapy was analyzed using whole-exome sequencing. The most frequent variants at diagnosis and at relapse comprised six genes: TP53, ATM, KMT2D, CCND1, SP140, and LRP1B. The most frequent CNVs at diagnosis and at relapse included TP53 and CDKN2A/B deletions, and PIK3CA amplifications. The mean count of mutations per patient significantly increased at relapse (n = 34) compared to diagnosis (n = 27). The most frequent newly detected variants at relapse, LRP1B gene mutations, correlated with a higher mutational burden. Variant allele frequencies of TP53 variants increased from 0.35 to 0.76 at relapse. The frequency and length of predicted CNVs significantly increased at relapse with CDKN2A/B deletions being the most frequent. Our data suggest, that the resistant MCL clones detected at relapse were already present at diagnosis and were selected by therapy. We observed enrichment of genetic aberrations of DNA damage response pathway (TP53 and CDKN2A/B), and a significant increase in MCL heterogeneity. We identified LRP1B inactivation as a new potential driver of MCL relapse.
Klíčová slova
sequencing-based analysis, clonal evolution, mantle cell lymphoma, failure of standard immunochemotherapy
Trvalý odkaz
https://hdl.handle.net/20.500.14178/2001Licence
Licence pro užití plného textu výsledku: Creative Commons Uveďte původ 4.0 International