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Loss of ADAR1 protein induces changes in small RNA landscape in hepatocytes

dc.contributor.authorRoučová, Kristina
dc.contributor.authorVopálenský, Václav
dc.contributor.authorMašek, Tomáš
dc.contributor.authordel Llano Solanas, Edgar
dc.contributor.authorProvazník, Jan
dc.contributor.authorLandry, Jonathan J. M.
dc.contributor.authorAzevedo, Nayara
dc.contributor.authorEhler, Edvard
dc.contributor.authorBeneš, Vladimír
dc.contributor.authorPospíšek, Martin
dc.date.accessioned2024-09-04T13:29:26Z
dc.date.available2024-09-04T13:29:26Z
dc.date.issued2024
dc.identifier.urihttps://hdl.handle.net/20.500.14178/2601
dc.description.abstractIn recent years, numerous evidence has been accumulated about the extent of A-to-I editing in human RNAs and the key role ADAR1 plays in the cellular editing machinery. It has been shown that A-to-I editing occurrence and frequency are tissue specific and essential for some tissue development, such as liver. To study the effect of ADAR1 function in hepatocytes, we have created Huh7.5 ADAR1 KO cell lines. Upon IFN treatment, the Huh7.5 ADAR1 KO cells show rapid arrest of growth and translation, from which they do not recover. We analyzed translatome changes by employing a method based on sequencing of separate polysome profile RNA fractions. We found significant changes in transcriptome and translatome of the Huh7.5 ADAR1 KO cells. The most prominent changes include negatively affected transcription by RNA polymerase III and the deregulation of snoRNA and Y RNA levels. Furthermore, we observed that ADAR1 KO polysomes are enriched in mRNAs coding for proteins pivotal in a wide range of biological processes such as RNA localization and RNA processing, whereas the unbound fraction is enriched mainly in mRNAs coding for ribosomal proteins and translational factors. This indicates that ADAR1 plays more relevant role in small RNA metabolism and ribosome biogenesis.en
dc.language.isoen
dc.relation.urlhttps://doi.org/10.1261/rna.080097.124
dc.rightsCreative Commons Uveďte původ-Neužívejte dílo komerčně 4.0 Internationalcs
dc.rightsCreative Commons Attribution-NonCommercial 4.0 Internationalen
dc.titleLoss of ADAR1 protein induces changes in small RNA landscape in hepatocytesen
dcterms.accessRightsopenAccess
dcterms.licensehttps://creativecommons.org/licenses/by-nc/4.0/legalcode
dc.date.updated2025-02-03T14:40:58Z
dc.subject.keywordADAR1 RNA editingen
dc.subject.keywordY RNAen
dc.subject.keywordhepatocyteen
dc.subject.keywordmiRNAen
dc.subject.keywordsnoRNAen
dc.identifier.eissn1469-9001
dc.relation.fundingReferenceinfo:eu-repo/grantAgreement/MSM//LX22NPO5103
dc.relation.fundingReferenceinfo:eu-repo/grantAgreement/MSM//SVV260679
dc.relation.fundingReferenceinfo:eu-repo/grantAgreement/UK/COOP/COOP
dc.date.embargoStartDate2025-02-03
dc.type.obd73
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1261/rna.080097.124
dc.identifier.utWos001293563700001
dc.identifier.eidScopus2-s2.0-85201727703
dc.identifier.obd649021
dc.identifier.pubmed38844344
dc.subject.rivPrimary10000::10600
dcterms.isPartOf.nameRNA
dcterms.isPartOf.issn1355-8382
dcterms.isPartOf.journalYear2024
dcterms.isPartOf.journalVolume30
dcterms.isPartOf.journalIssue9
uk.faculty.primaryId115
uk.faculty.primaryNamePřírodovědecká fakultacs
uk.faculty.primaryNameFaculty of Scienceen
uk.faculty.secondaryId117
uk.faculty.secondaryNamePedagogická fakultacs
uk.faculty.secondaryNameFaculty of Educationen
uk.department.primaryId1034
uk.department.primaryNameKatedra genetiky a mikrobiologiecs
uk.department.primaryNameDepartment of Genetics and Microbiologyen
uk.department.secondaryId1569
uk.department.secondaryNameKatedra biologie a environmentálních studiícs
uk.department.secondaryNameDepartment of Biology and Environmental Studiesen
dc.description.pageRange1164-1183
dc.type.obdHierarchyCsČLÁNEK V ČASOPISU::článek v časopisu::původní článekcs
dc.type.obdHierarchyEnJOURNAL ARTICLE::journal article::original articleen
dc.type.obdHierarchyCode73::152::206en
uk.displayTitleLoss of ADAR1 protein induces changes in small RNA landscape in hepatocytesen


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