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Relationship between yeast virus like elements and poxviruses at the transcriptional level

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Author
Vopálenský, VáclavORCiD Profile - 0000-0002-4858-4645WoS Profile - Q-6418-2017Scopus Profile - 8575785500
Sýkora, Michal
Mělková, ZoraORCiD Profile - 0000-0002-9709-2110WoS Profile - J-4209-2017
Barvík, Ivan
Mašek, TomášORCiD Profile - 0000-0001-8732-0565WoS Profile - Q-4636-2017Scopus Profile - 6603131017
Horáčková, Kamila
Pospíšek, MartinORCiD Profile - 0000-0002-9490-8911WoS Profile - A-9100-2008Scopus Profile - 6602708932

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Publication date
2024
Published in
Czech Chemical Society Symposium Series
Volume / Issue
22 (6)
ISBN / ISSN
ISSN: 2336-7202
ISBN / ISSN
eISSN: 2336-7210
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  • 1. Faculty of Medicine
  • Faculty of Science
Abstract
Yeast cytoplasmic double-stranded DNA virus-like elements (VLEs, also known as linear plasmids) were found in a number of yeast species belonging to nine genera. The Kluyveromyces lactis pGKL1/2 plasmids, which serve as archetypes of yeast linear plasmids, are peculiar in many respects. Both plasmids are cytoplasmically localized, possess proteins covalently linked to their terminal inverted repeats and their compact genomes code for 15 genes in total including a killer toxin, two DNA polymerases, an RNA polymerase, a DNA helicase and a capping enzyme.Functions of most of the genes are putative and have not been assigned to them experimentally yet. We and others have found, that the RNA polymerase encoded by pGKL plasmids, as well as the capping enzyme and RNA helicase, are similar to the corresponding enzyme encoded by vaccinia virus (VACV). We have recently described unique structures of yeast VLEs mRNAs, we found that although these VLEs encode their own putative capping enzyme, only a few VLEs genes code for 5'-capped transcripts and that all of the VLE specific mRNAs are not 3'-polyadenylated. Surprisingly, the majority of VLE promoters give rise to uncapped mRNAs starting with short poly(A) sequences at their 5' ends that are not complementary to the plasmid DNA.
Keywords
Yeast, RNA, VLEs, virus
Permanent link
https://hdl.handle.net/20.500.14178/2656
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Full text of this result is licensed under: Creative Commons Uveďte původ 4.0 International

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